prl-simian virus 40 (sv40) Search Results


90
Promega control plasmid prl-sv40
Control Plasmid Prl Sv40, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega simian virus 40 reporter vector
Simian Virus 40 Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega prl-cytomegalovirus (cmv)luc vector
Prl Cytomegalovirus (Cmv)luc Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prl-cytomegalovirus (cmv)luc vector - by Bioz Stars, 2026-10
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Promega cdk9 negative-control sirna
Cdk9 Negative Control Sirna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega firefly luciferase (fl) construct
Firefly Luciferase (Fl) Construct, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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firefly luciferase (fl) construct - by Bioz Stars, 2026-10
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Promega prl-tk
Prl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega prl-cmv
Prl Cmv, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega plasmid prl/null
Plasmid Prl/Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega renilla luciferase control
DCNL1 modulates HIF1α activity. (A) HEK293 cells were transfected with nontargeting scrambled siRNA (siSCR) or DCNL1-targeting siRNA (siDCNL1). An equivalent amount of protein lysate was resolved by SDS-PAGE and detected by immunoblotting using the indicated antibodies. (B) Stable cell lines in the indicated cellular backgrounds were generated through lentivirus-mediated infection of two different shRNA constructs targeting DCNL1. Cells were treated with MG132 for 4 h and lysed, and an equivalent amount of protein lysate was resolved by SDS-PAGE. The indicated proteins were detected by immunoblotting. (C) The indicated stable cell lines were transiently transfected with plasmids encoding firefly <t>luciferase</t> under the control of a hypoxia response element and a <t>Renilla</t> luciferase control. Cells were treated with MG132 for 4 h prior to a dual-luciferase assay. pGIPZ was arbitrarily set to 100 to represent normal HIF1α activity. Error bars represent standard deviations of the mean. An unpaired t test was performed to assess the statistical significance.
Renilla Luciferase Control, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-simian+virus+40+(sv40)/renilla+control+vector/pmc03624248-67-26-25
Average 90 stars, based on 1 article reviews
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Promega dual luciferase assay
DCNL1 modulates HIF1α activity. (A) HEK293 cells were transfected with nontargeting scrambled siRNA (siSCR) or DCNL1-targeting siRNA (siDCNL1). An equivalent amount of protein lysate was resolved by SDS-PAGE and detected by immunoblotting using the indicated antibodies. (B) Stable cell lines in the indicated cellular backgrounds were generated through lentivirus-mediated infection of two different shRNA constructs targeting DCNL1. Cells were treated with MG132 for 4 h and lysed, and an equivalent amount of protein lysate was resolved by SDS-PAGE. The indicated proteins were detected by immunoblotting. (C) The indicated stable cell lines were transiently transfected with plasmids encoding firefly <t>luciferase</t> under the control of a hypoxia response element and a <t>Renilla</t> luciferase control. Cells were treated with MG132 for 4 h prior to a dual-luciferase assay. pGIPZ was arbitrarily set to 100 to represent normal HIF1α activity. Error bars represent standard deviations of the mean. An unpaired t test was performed to assess the statistical significance.
Dual Luciferase Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-simian+virus+40+(sv40)/luciferase+assay+system/10__1128_slash_mcb__01237___09-67-6-8
Average 90 stars, based on 1 article reviews
dual luciferase assay - by Bioz Stars, 2026-10
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Promega prl-cmv plasmid
DCNL1 modulates HIF1α activity. (A) HEK293 cells were transfected with nontargeting scrambled siRNA (siSCR) or DCNL1-targeting siRNA (siDCNL1). An equivalent amount of protein lysate was resolved by SDS-PAGE and detected by immunoblotting using the indicated antibodies. (B) Stable cell lines in the indicated cellular backgrounds were generated through lentivirus-mediated infection of two different shRNA constructs targeting DCNL1. Cells were treated with MG132 for 4 h and lysed, and an equivalent amount of protein lysate was resolved by SDS-PAGE. The indicated proteins were detected by immunoblotting. (C) The indicated stable cell lines were transiently transfected with plasmids encoding firefly <t>luciferase</t> under the control of a hypoxia response element and a <t>Renilla</t> luciferase control. Cells were treated with MG132 for 4 h prior to a dual-luciferase assay. pGIPZ was arbitrarily set to 100 to represent normal HIF1α activity. Error bars represent standard deviations of the mean. An unpaired t test was performed to assess the statistical significance.
Prl Cmv Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-simian+virus+40+(sv40)/prl+cmv+plasmid/10__1128_slash_mcb__06267___11-124-17-19
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Promega plasmid phrl-cmv
DCNL1 modulates HIF1α activity. (A) HEK293 cells were transfected with nontargeting scrambled siRNA (siSCR) or DCNL1-targeting siRNA (siDCNL1). An equivalent amount of protein lysate was resolved by SDS-PAGE and detected by immunoblotting using the indicated antibodies. (B) Stable cell lines in the indicated cellular backgrounds were generated through lentivirus-mediated infection of two different shRNA constructs targeting DCNL1. Cells were treated with MG132 for 4 h and lysed, and an equivalent amount of protein lysate was resolved by SDS-PAGE. The indicated proteins were detected by immunoblotting. (C) The indicated stable cell lines were transiently transfected with plasmids encoding firefly <t>luciferase</t> under the control of a hypoxia response element and a <t>Renilla</t> luciferase control. Cells were treated with MG132 for 4 h prior to a dual-luciferase assay. pGIPZ was arbitrarily set to 100 to represent normal HIF1α activity. Error bars represent standard deviations of the mean. An unpaired t test was performed to assess the statistical significance.
Plasmid Phrl Cmv, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-simian+virus+40+(sv40)/plasmid+phrl+cmv/10__1128_slash_jvi__78__11__5679___5685__2004-65-0-2
Average 90 stars, based on 1 article reviews
plasmid phrl-cmv - by Bioz Stars, 2026-10
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Image Search Results


DCNL1 modulates HIF1α activity. (A) HEK293 cells were transfected with nontargeting scrambled siRNA (siSCR) or DCNL1-targeting siRNA (siDCNL1). An equivalent amount of protein lysate was resolved by SDS-PAGE and detected by immunoblotting using the indicated antibodies. (B) Stable cell lines in the indicated cellular backgrounds were generated through lentivirus-mediated infection of two different shRNA constructs targeting DCNL1. Cells were treated with MG132 for 4 h and lysed, and an equivalent amount of protein lysate was resolved by SDS-PAGE. The indicated proteins were detected by immunoblotting. (C) The indicated stable cell lines were transiently transfected with plasmids encoding firefly luciferase under the control of a hypoxia response element and a Renilla luciferase control. Cells were treated with MG132 for 4 h prior to a dual-luciferase assay. pGIPZ was arbitrarily set to 100 to represent normal HIF1α activity. Error bars represent standard deviations of the mean. An unpaired t test was performed to assess the statistical significance.

Journal: Molecular and Cellular Biology

Article Title: DCNL1 Functions as a Substrate Sensor and Activator of Cullin 2-RING Ligase

doi: 10.1128/MCB.01342-12

Figure Lengend Snippet: DCNL1 modulates HIF1α activity. (A) HEK293 cells were transfected with nontargeting scrambled siRNA (siSCR) or DCNL1-targeting siRNA (siDCNL1). An equivalent amount of protein lysate was resolved by SDS-PAGE and detected by immunoblotting using the indicated antibodies. (B) Stable cell lines in the indicated cellular backgrounds were generated through lentivirus-mediated infection of two different shRNA constructs targeting DCNL1. Cells were treated with MG132 for 4 h and lysed, and an equivalent amount of protein lysate was resolved by SDS-PAGE. The indicated proteins were detected by immunoblotting. (C) The indicated stable cell lines were transiently transfected with plasmids encoding firefly luciferase under the control of a hypoxia response element and a Renilla luciferase control. Cells were treated with MG132 for 4 h prior to a dual-luciferase assay. pGIPZ was arbitrarily set to 100 to represent normal HIF1α activity. Error bars represent standard deviations of the mean. An unpaired t test was performed to assess the statistical significance.

Article Snippet: Cells were transfected in triplicate with pGL3-5×HRE-Luc (where Luc is luciferase) (kind gift from Richard P. Hill) and pRL-SV40 (where SV40 is simian virus 40) (Promega) Renilla luciferase control.

Techniques: Activity Assay, Transfection, SDS Page, Western Blot, Stable Transfection, Generated, Infection, shRNA, Construct, Luciferase, Control